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Open Access Article

Organic Chemistry and Synthesis. 2025; 1: (1) ; 12-15 ; DOI: 10.12208/j.ocs.20250003.

During fermentation, microbiology and biochemistry of the cocoa bean
可可豆发酵过程中的微生物学和生物化学

作者: Mulono Apriyanto *, Rifni Novitasari

因德拉吉里伊斯兰大学农学院食品技术系 印度尼西亚

*通讯作者: Mulono Apriyanto,单位:因德拉吉里伊斯兰大学农学院食品技术系 印度尼西亚;

发布时间: 2025-06-12 总浏览量: 21

摘要

本研究旨在定量和定性地检测干可可豆的发酵参数,以更有效地确定微生物的作用和功能。三种发酵方法如下:对照组(不接种)、酿酒酵母(FNCC接种物3056)、乳酸乳杆菌(FNC0086)和醋酸杆菌(FNCC0016),浓度均为约108CFU/g,在发酵开始时同时添加(A);三种接种物分阶段添加(B)。结果表明,在发酵过程中,三种处理均降低了总糖含量、pH值和总多酚含量。在所有处理中,乙醇、乳酸和乙酸的浓度分别在发酵24、60和108小时时达到峰值。在发酵24、48和72小时时,从三种处理中获得了最多的酿酒酵母、乳酸杆菌和醋酸杆菌数量。

关键词: 微生物;酿酒酵母;乳酸杆菌;醋酸杆菌;发酵和干燥可可豆

Abstract

The purpose of this research is to quantitatively and qualitatively examine the fermentation parameters of dry cocoa beans to be more effective in determining the role and function of microorganisms. The three fermentation methods are as follows: control (without inoculums), Saccharomyces cerevisiae (FNCC inoculums 3056), Lactobacillus lactis (FNC 0086), and Acetobacter aceti (FNCC 0016), all at a concentration of approximately 108 CFU/g were added simultaneously at the start of the fermentation (A), and all three inoculums were added in stages (B). The results indicated that during fermentation, all three treatments decreased total sugar content, pH, and total polyphenols. In all treatments, the concentrations of ethanol, lactic acid, and acetic acid peaked at 24, 60, and 108 hours of fermentation, respectively. At 24, 48, and 72 hours of fermentation, the highest populations of Saccharomyces cerevisiae, Lactobacillus lactis, and Acetobacter aceti were obtained from the three treatments.

Key words: Microbes; Saccharomyces cerevisiae; Lactobacillus lactis; Acetobacter aceti; Fermentation and dried cocoa beans

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引用本文

MulonoApriyanto, RifniNovitasari, 可可豆发酵过程中的微生物学和生物化学[J]. 有机化学与合成, 2025; 1: (1) : 12-15.